Division of Virology

Division of Virology, within the Department of Microbiology

Information about the activity performed in Division of Virology

The Department of Virology and Molecular Diagnostics is the only one of its kind in the Republic of North Macedonia. It was founded for the first time in 1960. with the aim of: monitoring, study and laboratory diagnosis of viral diseases. At the same time, the most modern serological (immunoenzyme and immunoblot) and molecular diagnostic methods are applied, as well as the isolation of viruses on cell cultures, while following the world literature and WHO recommendations.

Since 2005 the department is accredited by the WHO for the National Laboratory for Measles and Rubella in the Republic of North Macedonia. Since 2006 The HIV Laboratory has been designated as the Reference Laboratory for HIV/AIDS in R. North Macedonia, within which there is also a counseling center for free testing and counseling. The Influenza Laboratory has been nominated by the Ministry of Health as a National Influenza Center and is included in the WHO Program for External Quality Assessment for Influenza Virus Detection. On January 17, 2020, the diagnosis of the pandemic SARS-CoV-2 (Novel corona virus 2019-nCoV) was introduced for the first time in the country. The department is the only one in the country charged by the WHO in the process of maintaining the status of a country free from poliomyelitis.


Measures and activities under the competence of the Division of Virology

Analyzes performed in the laboratory:

Detection of RNA/DNA by RT PCR of the following viruses:

  1. Human Adenovirus A/B
  2. Coronavirus 229E
  3. Coronavirus HKU1
  4. Coronavirus NL63
  5. Coronavirus OC43
  6. MERC
  7. CoV
  8. SARS CoV-2
  9. Human Metapneumovirus
  10. Human Rhinovirus/Enterovirus
  11. Influenza A
  12. Influenza B
  13. Parainfluenza virus1
  14. Parainfluenza virus2
  15. Parainfluenza virus3
  16. Parainfluenza virus4
  17. Respiratory syncytial virus
  18. Morbilli virus
  19. Rubella virus
  20. West Nile virus
  21. Dengue virus
  22. CHIKU virus
  23. Zika virus
  24. CMV
  25. Epstein Bar virus
  26. Human Herpes virus 6
  27. Varicella Zoster virus
  28. Human Herpes virus 1
  29. Human Herpes virus 2
  30. Human monkeypox virus (MPXV) 36
  31. subtyping of influenza viruses Influenza A and B.
  32. detection and characterization of the VOC and VOI variants of the SARS-CoV-2 virus.

Since January 2021, sequencing of the entire genome of the SARS-CoV-2 virus, as well as the rest of the viruses from the respiratory panel, has been underway. From July 2022, detection of MPXV as well as sequencing of the genome of Monkeypox virus is underway. Detection of antigen or antibodies to viruses with:

  1. ELISA – Enzyme immunoassay
  2. ELFA – Enzyme Immuno Fluorescence Assay
  3. IEHT – Immuno Enzyme Chemiluminescent Test
  4. IHT – Immunochromatographic test
  5. WB - Western blot test - Cytomegalo virus (CMV) IgM blood
  6. Cytomegalo virus (CMV) IgG blood
  7. Cytomegalo virus (CMV) IgGAvidity-determining the age of blood antibodies
  8. HAV IgM blood antibodies - HAV Total blood antibodies
  9. HBs antigen blood - HBs total antibodies blood
  10. HBc total blood antibodies
  11. HBc IgM antibodies blood
  12. HBe antigen blood
  13. HBe total blood antibodies
  14. HCV antibodies blood
  15. HCV RNA – quantitative blood
  16. HIV 1/2 antibodies blood
  17. HIV integral (Ag +At) blood
  18. Herpes simplex virus (HSV2) IgM antibodies blood
  19. Herpes simplex virus (HSV2) IgG antibodies blood
  20. Herpes simplex virus (HSV1) IgM antibodies blood
  21. Herpes simplex virus (HSV1) IgG antibodies blood
  22. Herpes zoster virus – Varicella (VZV) IgM antibodies blood
  23. Herpes zoster virus – Varicella (VZV) IgG antibodies blood
  24. Morbilli IgM antibodies blood - Morbilli IgG antibodies blood
  25. Mumps (parotitis) IgM antibodies blood
  26. Mumps (parotitis) IgG antibodies blood
  27. Rubella IgM antibodies blood
  28. Rubella IgG antibodies blood
  29. West –Nile virus IgM antibodies blood
  30. West –Nile virus IgG antibodies blood
  31. Hantaan virus IgM antibodies blood
  32. Hantaan virus IgG antibodies blood
  33. TBE virus IgM antibodies blood
  34. TBE virus IgG antibodies blood
  35. Congo Crimean Haemorrhagic Fever virus IgM antibodies blood
  36. Congo Crimean Haemorrhagic Fever virus IgG antibodies blood
  37. Parvovirus B19 IgM antibodies blood
  38. Parvovirus B19 Ig G antibodies blood
  39. Entero viruses ( Rota, Noro, Astro, Adeno) feces.

The Department also performs immunoenzymatic tests to prove antibodies against the following microorganisms:

  1. Borrelia burgdorferi (Lyme borreliosis) IgM antibodies blood
  2. Borrelia burgdorferi (Lyme borreliosis) IgG antibodies blood
  3. Toxoplasma gondii IgM antibodies blood - Toxoplasma gondii IgG antibodies blood
  4. Toxoplasma gondii IgG Avidity–determining the age of Antibodies blood
  5. Q fever phase ½ IgM antibodies blood
  6. Q fever phase ½ IgG antibodies blood
  7. Helicobacter pylori IgG antibodies blood.

Contact information

Contact person: Prof. Golubinka Boshevska, MD, Phd

Contact phone: 02 312 50 44 лок. 43

Email golubinka@yahoo.com